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Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem
Sweet perception promotes food intake, whereas that of bitterness is inhibitory. Surprisingly, the expression of sweet G protein-coupled taste receptor (GPCTR) subunits (T1R2 and T1R3) and bitter GPCTRs (T2R116, T2R118, T2R138 and T2R104), as well as the α-subunits of the associated signalling compl...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937374/ https://www.ncbi.nlm.nih.gov/pubmed/27388805 http://dx.doi.org/10.1038/srep29094 |
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author | Herrera Moro Chao, D. Argmann, C. Van Eijk, M. Boot, R. G. Ottenhoff, R. Van Roomen, C. Foppen, E. Siljee, J. E. Unmehopa, U. A. Kalsbeek, A. Aerts, J. M. F. G. |
author_facet | Herrera Moro Chao, D. Argmann, C. Van Eijk, M. Boot, R. G. Ottenhoff, R. Van Roomen, C. Foppen, E. Siljee, J. E. Unmehopa, U. A. Kalsbeek, A. Aerts, J. M. F. G. |
author_sort | Herrera Moro Chao, D. |
collection | PubMed |
description | Sweet perception promotes food intake, whereas that of bitterness is inhibitory. Surprisingly, the expression of sweet G protein-coupled taste receptor (GPCTR) subunits (T1R2 and T1R3) and bitter GPCTRs (T2R116, T2R118, T2R138 and T2R104), as well as the α-subunits of the associated signalling complex (αGustducin, Gα14 and αTransducin), in oral and extra-oral tissues from lean and obese mice, remains poorly characterized. We focused on the impact of obesity on taste receptor expression in brain areas involved in energy homeostasis, namely the hypothalamus and brainstem. We demonstrate that many of the GPCTRs and α-subunits are co-expressed in these tissues and that obesity decreases expression of T1R3, T2R116, Gα14, αTrans and TRPM5. In vitro high levels of glucose caused a prominent down-regulation of T1R2 and Gα14 expression in cultured hypothalamic neuronal cells, leptin caused a transient down-regulation of T1R2 and T1R3 expression. Intriguingly, expression differences were also observed in other extra-oral tissues of lean and obese mice, most strikingly in the duodenum where obesity reduced the expression of most bitter and sweet receptors. In conclusion, obesity influences components of sweet and bitter taste sensing in the duodenum as well as regions of the mouse brain involved in energy homeostasis, including hypothalamus and brainstem. |
format | Online Article Text |
id | pubmed-4937374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49373742016-07-13 Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem Herrera Moro Chao, D. Argmann, C. Van Eijk, M. Boot, R. G. Ottenhoff, R. Van Roomen, C. Foppen, E. Siljee, J. E. Unmehopa, U. A. Kalsbeek, A. Aerts, J. M. F. G. Sci Rep Article Sweet perception promotes food intake, whereas that of bitterness is inhibitory. Surprisingly, the expression of sweet G protein-coupled taste receptor (GPCTR) subunits (T1R2 and T1R3) and bitter GPCTRs (T2R116, T2R118, T2R138 and T2R104), as well as the α-subunits of the associated signalling complex (αGustducin, Gα14 and αTransducin), in oral and extra-oral tissues from lean and obese mice, remains poorly characterized. We focused on the impact of obesity on taste receptor expression in brain areas involved in energy homeostasis, namely the hypothalamus and brainstem. We demonstrate that many of the GPCTRs and α-subunits are co-expressed in these tissues and that obesity decreases expression of T1R3, T2R116, Gα14, αTrans and TRPM5. In vitro high levels of glucose caused a prominent down-regulation of T1R2 and Gα14 expression in cultured hypothalamic neuronal cells, leptin caused a transient down-regulation of T1R2 and T1R3 expression. Intriguingly, expression differences were also observed in other extra-oral tissues of lean and obese mice, most strikingly in the duodenum where obesity reduced the expression of most bitter and sweet receptors. In conclusion, obesity influences components of sweet and bitter taste sensing in the duodenum as well as regions of the mouse brain involved in energy homeostasis, including hypothalamus and brainstem. Nature Publishing Group 2016-07-08 /pmc/articles/PMC4937374/ /pubmed/27388805 http://dx.doi.org/10.1038/srep29094 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Herrera Moro Chao, D. Argmann, C. Van Eijk, M. Boot, R. G. Ottenhoff, R. Van Roomen, C. Foppen, E. Siljee, J. E. Unmehopa, U. A. Kalsbeek, A. Aerts, J. M. F. G. Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem |
title | Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem |
title_full | Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem |
title_fullStr | Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem |
title_full_unstemmed | Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem |
title_short | Impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem |
title_sort | impact of obesity on taste receptor expression in extra-oral tissues: emphasis on hypothalamus and brainstem |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937374/ https://www.ncbi.nlm.nih.gov/pubmed/27388805 http://dx.doi.org/10.1038/srep29094 |
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