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Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage
Lineage-specific transcription factors determine the cell fate during development. Direct conversion of several cell types into other lineages has been achieved by the overexpression of specific transcription factors. Even cancer cells have been demonstrated to be amenable to transdifferentiation. H...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937495/ https://www.ncbi.nlm.nih.gov/pubmed/27387763 http://dx.doi.org/10.1038/srep28891 |
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author | Fehrenbach, Sabrina Novak, Daniel Bernhardt, Mathias Larribere, Lionel Boukamp, Petra Umansky, Viktor Utikal, Jochen |
author_facet | Fehrenbach, Sabrina Novak, Daniel Bernhardt, Mathias Larribere, Lionel Boukamp, Petra Umansky, Viktor Utikal, Jochen |
author_sort | Fehrenbach, Sabrina |
collection | PubMed |
description | Lineage-specific transcription factors determine the cell fate during development. Direct conversion of several cell types into other lineages has been achieved by the overexpression of specific transcription factors. Even cancer cells have been demonstrated to be amenable to transdifferentiation. Here, we identified a distinct set of transcription factors, which are sufficient to transform cells of the keratinocytic lineage to melanocyte-like cells. Melanocyte marker expression was induced and melanosome formation was observed in non-tumorigenic keratinocytes (HaCaT) and tumorigenic squamous cell carcinoma (MET-4) cells. Moreover, reduced proliferation, cell metabolism, invasion and migration were measured in vitro in transdifferentiated MT-MET-4 cells. A loss of tumorigenic potential of squamous cell carcinoma cells could be due to the upregulation of the melanocyte differentiation associated gene IL-24. Our data show that cells from the keratinocytic lineage can be transdifferented into the melanocytic lineage and provide a proof of principle for a potential new therapeutic strategy. |
format | Online Article Text |
id | pubmed-4937495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49374952016-07-18 Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage Fehrenbach, Sabrina Novak, Daniel Bernhardt, Mathias Larribere, Lionel Boukamp, Petra Umansky, Viktor Utikal, Jochen Sci Rep Article Lineage-specific transcription factors determine the cell fate during development. Direct conversion of several cell types into other lineages has been achieved by the overexpression of specific transcription factors. Even cancer cells have been demonstrated to be amenable to transdifferentiation. Here, we identified a distinct set of transcription factors, which are sufficient to transform cells of the keratinocytic lineage to melanocyte-like cells. Melanocyte marker expression was induced and melanosome formation was observed in non-tumorigenic keratinocytes (HaCaT) and tumorigenic squamous cell carcinoma (MET-4) cells. Moreover, reduced proliferation, cell metabolism, invasion and migration were measured in vitro in transdifferentiated MT-MET-4 cells. A loss of tumorigenic potential of squamous cell carcinoma cells could be due to the upregulation of the melanocyte differentiation associated gene IL-24. Our data show that cells from the keratinocytic lineage can be transdifferented into the melanocytic lineage and provide a proof of principle for a potential new therapeutic strategy. Nature Publishing Group 2016-07-08 /pmc/articles/PMC4937495/ /pubmed/27387763 http://dx.doi.org/10.1038/srep28891 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Fehrenbach, Sabrina Novak, Daniel Bernhardt, Mathias Larribere, Lionel Boukamp, Petra Umansky, Viktor Utikal, Jochen Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage |
title | Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage |
title_full | Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage |
title_fullStr | Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage |
title_full_unstemmed | Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage |
title_short | Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage |
title_sort | loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937495/ https://www.ncbi.nlm.nih.gov/pubmed/27387763 http://dx.doi.org/10.1038/srep28891 |
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