An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

The plasmid vector pGreenII is widely used to produce plant transformants via a process that involves propagation in Escherichia coli. However, we show here that pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death. In additio...

Descripción completa

Detalles Bibliográficos
Autores principales: Watson, Michael R., Lin, Yu-fei, Hollwey, Elizabeth, Dodds, Rachel E., Meyer, Peter, McDowall, Kenneth J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938672/
https://www.ncbi.nlm.nih.gov/pubmed/27194805
http://dx.doi.org/10.1534/g3.116.029405

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938672/
https://www.ncbi.nlm.nih.gov/pubmed/27194805
http://dx.doi.org/10.1534/g3.116.029405

Ejemplares similares