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Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster
Transformation-associated recombination (TAR) in yeast is a rapid and inexpensive method for cloning and assembly of large DNA fragments, which relies on natural homologous recombination. Two vectors, based on p15a and F-factor replicons that can be maintained in yeast, E. coli and streptomycetes ha...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943663/ https://www.ncbi.nlm.nih.gov/pubmed/27410036 http://dx.doi.org/10.1371/journal.pone.0158682 |