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Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster

Transformation-associated recombination (TAR) in yeast is a rapid and inexpensive method for cloning and assembly of large DNA fragments, which relies on natural homologous recombination. Two vectors, based on p15a and F-factor replicons that can be maintained in yeast, E. coli and streptomycetes ha...

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Detalles Bibliográficos
Autores principales:	Bilyk, Oksana, Sekurova, Olga N., Zotchev, Sergey B., Luzhetskyy, Andriy
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943663/ https://www.ncbi.nlm.nih.gov/pubmed/27410036 http://dx.doi.org/10.1371/journal.pone.0158682

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