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Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation

Efficient islet isolation requires synergistic interaction between collagenase class I (CI) and class II (CII). The CI degradation alters the ratio between CI and CII and is responsible for batch-to-batch variations. This study compares the role of neutral protease (NP) plus clostripain (CP) with CI...

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Detalles Bibliográficos
Autores principales: Brandhorst, Heide, Kurfürst, Manfred, Johnson, Paul R., Korsgren, Olle, Brandhorst, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946504/
https://www.ncbi.nlm.nih.gov/pubmed/27500241
http://dx.doi.org/10.1097/TXD.0000000000000552