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The effect of injection speed and serial injection on propidium iodide entry into cultured HeLa and primary neonatal fibroblast cells using lance array nanoinjection

BACKGROUND: Although site-directed genetic engineering has greatly improved in recent years, particularly with the implementation of CRISPR-Cas9, the ability to deliver these molecular constructs to a wide variety of cell types without adverse reaction is still a challenge. One non-viral transfectio...

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Detalles Bibliográficos
Autores principales: Sessions, John W., Lewis, Tyler E., Skousen, Craig S., Hope, Sandra, Jensen, Brian D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947087/
https://www.ncbi.nlm.nih.gov/pubmed/27468394
http://dx.doi.org/10.1186/s40064-016-2757-5