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Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics

In order to exploit the potential benefits of antimicrobial combination therapy, we need a better understanding of the circumstances under which pharmacodynamic interactions expected. In this study, Pharmacodynamic interactions between silver nanoparticle (SNP) and topical antibiotics such as Cefazo...

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Autores principales: Thirumurugan, G., Seshagiri Rao, J. V. L. N., Dhanaraju, M. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947953/
https://www.ncbi.nlm.nih.gov/pubmed/27427207
http://dx.doi.org/10.1038/srep29982
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author Thirumurugan, G.
Seshagiri Rao, J. V. L. N.
Dhanaraju, M. D.
author_facet Thirumurugan, G.
Seshagiri Rao, J. V. L. N.
Dhanaraju, M. D.
author_sort Thirumurugan, G.
collection PubMed
description In order to exploit the potential benefits of antimicrobial combination therapy, we need a better understanding of the circumstances under which pharmacodynamic interactions expected. In this study, Pharmacodynamic interactions between silver nanoparticle (SNP) and topical antibiotics such as Cefazolin (CEF), Mupirocin (MUP), Gentamycin (GEN), Neomycin (NEO), Tetracycline (TET), Vancomycin (VAN) were investigated using the MIC test, Combination assay followed by Fractional Inhibitory concentration Index and Agar well diffusion method. SNP + MUP, SNP + NEO, SNP + VAN combinations showed Synergism (SN) and SNP + CEF, SNP + GEN, SNP + TET showed Partial synergism (PS) against Staphylococcus aureus. Four combinations (SNP + CEF, SNP + MUP, SNP + GEN, SNP + VAN) showed SN, SNP + TET showed PS and Indifferent effect (ID) were observed for SNP + NEO against Pseudomonas aeruginosa. SN was observed for SNP + CEF, SNP + GEN, SNP + NEO, SNP + TET and SNP + MUP showed ID, SNP + VAN showed PS against Escherichia coli. In addition, we elucidated the possible mechanism involved in the pharmacodynamic interaction between SNP-topical antibiotics by increased ROS level, membrane damage following protein release, K(+) leakage and biofilm inhibition. Thus, our findings support that conjugation of the SNP with topical antibiotics have great potential in the topical formulation when treating complex resistant bacterial infections and where there is a need of more concentration to kill pathogenic bacteria.
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spelling pubmed-49479532016-07-26 Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics Thirumurugan, G. Seshagiri Rao, J. V. L. N. Dhanaraju, M. D. Sci Rep Article In order to exploit the potential benefits of antimicrobial combination therapy, we need a better understanding of the circumstances under which pharmacodynamic interactions expected. In this study, Pharmacodynamic interactions between silver nanoparticle (SNP) and topical antibiotics such as Cefazolin (CEF), Mupirocin (MUP), Gentamycin (GEN), Neomycin (NEO), Tetracycline (TET), Vancomycin (VAN) were investigated using the MIC test, Combination assay followed by Fractional Inhibitory concentration Index and Agar well diffusion method. SNP + MUP, SNP + NEO, SNP + VAN combinations showed Synergism (SN) and SNP + CEF, SNP + GEN, SNP + TET showed Partial synergism (PS) against Staphylococcus aureus. Four combinations (SNP + CEF, SNP + MUP, SNP + GEN, SNP + VAN) showed SN, SNP + TET showed PS and Indifferent effect (ID) were observed for SNP + NEO against Pseudomonas aeruginosa. SN was observed for SNP + CEF, SNP + GEN, SNP + NEO, SNP + TET and SNP + MUP showed ID, SNP + VAN showed PS against Escherichia coli. In addition, we elucidated the possible mechanism involved in the pharmacodynamic interaction between SNP-topical antibiotics by increased ROS level, membrane damage following protein release, K(+) leakage and biofilm inhibition. Thus, our findings support that conjugation of the SNP with topical antibiotics have great potential in the topical formulation when treating complex resistant bacterial infections and where there is a need of more concentration to kill pathogenic bacteria. Nature Publishing Group 2016-07-18 /pmc/articles/PMC4947953/ /pubmed/27427207 http://dx.doi.org/10.1038/srep29982 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Thirumurugan, G.
Seshagiri Rao, J. V. L. N.
Dhanaraju, M. D.
Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics
title Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics
title_full Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics
title_fullStr Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics
title_full_unstemmed Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics
title_short Elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics
title_sort elucidating pharmacodynamic interaction of silver nanoparticle - topical deliverable antibiotics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947953/
https://www.ncbi.nlm.nih.gov/pubmed/27427207
http://dx.doi.org/10.1038/srep29982
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