The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays

The complexities of DNA recognition by transcription factors (TFs) with multiple Cys2–His2 zinc fingers (C2H2-ZFs) remain poorly studied. We previously reported a mutation (R1092W) in the C2H2-ZF TF Zfp335 that led to selective loss of binding at a subset of targets, although the basis for this effe...

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Detalles Bibliográficos
Autores principales: Han, Brenda Yuyuan, Foo, Chuan-Sheng, Wu, Shuang, Cyster, Jason G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949324/
https://www.ncbi.nlm.nih.gov/pubmed/27401554
http://dx.doi.org/10.1101/gad.279406.116
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author Han, Brenda Yuyuan
Foo, Chuan-Sheng
Wu, Shuang
Cyster, Jason G.
author_facet Han, Brenda Yuyuan
Foo, Chuan-Sheng
Wu, Shuang
Cyster, Jason G.
author_sort Han, Brenda Yuyuan
collection PubMed
description The complexities of DNA recognition by transcription factors (TFs) with multiple Cys2–His2 zinc fingers (C2H2-ZFs) remain poorly studied. We previously reported a mutation (R1092W) in the C2H2-ZF TF Zfp335 that led to selective loss of binding at a subset of targets, although the basis for this effect was unclear. We show that Zfp335 binds DNA and drives transcription via recognition of two distinct consensus motifs by separate ZF clusters and identify the specific motif interaction disrupted by R1092W. Our work presents Zfp335 as a model for understanding how C2H2-ZF TFs may use multiple recognition motifs to control gene expression.
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spelling pubmed-49493242017-01-01 The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays Han, Brenda Yuyuan Foo, Chuan-Sheng Wu, Shuang Cyster, Jason G. Genes Dev Research Communication The complexities of DNA recognition by transcription factors (TFs) with multiple Cys2–His2 zinc fingers (C2H2-ZFs) remain poorly studied. We previously reported a mutation (R1092W) in the C2H2-ZF TF Zfp335 that led to selective loss of binding at a subset of targets, although the basis for this effect was unclear. We show that Zfp335 binds DNA and drives transcription via recognition of two distinct consensus motifs by separate ZF clusters and identify the specific motif interaction disrupted by R1092W. Our work presents Zfp335 as a model for understanding how C2H2-ZF TFs may use multiple recognition motifs to control gene expression. Cold Spring Harbor Laboratory Press 2016-07-01 /pmc/articles/PMC4949324/ /pubmed/27401554 http://dx.doi.org/10.1101/gad.279406.116 Text en © 2016 Han et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Han, Brenda Yuyuan
Foo, Chuan-Sheng
Wu, Shuang
Cyster, Jason G.
The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays
title The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays
title_full The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays
title_fullStr The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays
title_full_unstemmed The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays
title_short The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays
title_sort c2h2-zf transcription factor zfp335 recognizes two consensus motifs using separate zinc finger arrays
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949324/
https://www.ncbi.nlm.nih.gov/pubmed/27401554
http://dx.doi.org/10.1101/gad.279406.116
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