High-yield cell-free synthesis of human EGFR by IRES-mediated protein translation in a continuous exchange cell-free reaction format

Cell-free protein synthesis systems derived from eukaryotic sources often provide comparatively low amounts of several μg per ml of de novo synthesized membrane protein. In order to overcome this, we herein demonstrate the high-yield cell-free synthesis of the human EGFR in a microsome-containing sy...

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Detalles Bibliográficos
Autores principales: Quast, Robert B., Sonnabend, Andrei, Stech, Marlitt, Wüstenhagen, Doreen A., Kubick, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960648/
https://www.ncbi.nlm.nih.gov/pubmed/27456041
http://dx.doi.org/10.1038/srep30399

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960648/
https://www.ncbi.nlm.nih.gov/pubmed/27456041
http://dx.doi.org/10.1038/srep30399

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