Fast and Efficient Screening for Wheat Loss-of-Gene Mutants Using Multiplexed Melt Curve Analyses

This study describes a new approach in the screening for loss-of-gene mutants in Heavy Ion Bombardment (HIB) mutant populations of genetically complex organisms such as hexaploid bread wheat using multiplexed single-color (SYBR Green) melt curve analyses. The assay was set up for three target genes...

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Detalles Bibliográficos
Autores principales: Mieog, Jos C., Ral, Jean-Philippe F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961375/
https://www.ncbi.nlm.nih.gov/pubmed/27459606
http://dx.doi.org/10.1371/journal.pone.0159955

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961375/
https://www.ncbi.nlm.nih.gov/pubmed/27459606
http://dx.doi.org/10.1371/journal.pone.0159955

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