Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells

Galangin suppresses proliferation and induces apoptosis and autophagy in hepatocellular carcinoma (HCC) cells, but the precise mechanism is not clear. In this study, we demonstrated that galangin induced autophagy, enhanced the binding of SIRT1-LC3 and reduced the acetylation of endogenous LC3 in He...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xv, Wang, Yajun, Xiong, Yuzhen, Wu, Jun, Ding, Hang, Chen, Xiaoyi, Lan, Liubo, Zhang, Haitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962058/
https://www.ncbi.nlm.nih.gov/pubmed/27460655
http://dx.doi.org/10.1038/srep30496
_version_ 1782444759520903168
author Li, Xv
Wang, Yajun
Xiong, Yuzhen
Wu, Jun
Ding, Hang
Chen, Xiaoyi
Lan, Liubo
Zhang, Haitao
author_facet Li, Xv
Wang, Yajun
Xiong, Yuzhen
Wu, Jun
Ding, Hang
Chen, Xiaoyi
Lan, Liubo
Zhang, Haitao
author_sort Li, Xv
collection PubMed
description Galangin suppresses proliferation and induces apoptosis and autophagy in hepatocellular carcinoma (HCC) cells, but the precise mechanism is not clear. In this study, we demonstrated that galangin induced autophagy, enhanced the binding of SIRT1-LC3 and reduced the acetylation of endogenous LC3 in HepG2 cells. But this autophagy was inhibited by inactivation of SIRT1 meanwhile, galangin failed to reduce the acetylation of endogenous LC3 after SIRT1 was knocked-down. Collectively, these findings demonstrate a new mechanism by which galangin induces autophagy via the deacetylation of endogenous LC3 by SIRT1.
format Online
Article
Text
id pubmed-4962058
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-49620582016-08-08 Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells Li, Xv Wang, Yajun Xiong, Yuzhen Wu, Jun Ding, Hang Chen, Xiaoyi Lan, Liubo Zhang, Haitao Sci Rep Article Galangin suppresses proliferation and induces apoptosis and autophagy in hepatocellular carcinoma (HCC) cells, but the precise mechanism is not clear. In this study, we demonstrated that galangin induced autophagy, enhanced the binding of SIRT1-LC3 and reduced the acetylation of endogenous LC3 in HepG2 cells. But this autophagy was inhibited by inactivation of SIRT1 meanwhile, galangin failed to reduce the acetylation of endogenous LC3 after SIRT1 was knocked-down. Collectively, these findings demonstrate a new mechanism by which galangin induces autophagy via the deacetylation of endogenous LC3 by SIRT1. Nature Publishing Group 2016-07-27 /pmc/articles/PMC4962058/ /pubmed/27460655 http://dx.doi.org/10.1038/srep30496 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Xv
Wang, Yajun
Xiong, Yuzhen
Wu, Jun
Ding, Hang
Chen, Xiaoyi
Lan, Liubo
Zhang, Haitao
Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
title Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
title_full Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
title_fullStr Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
title_full_unstemmed Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
title_short Galangin Induces Autophagy via Deacetylation of LC3 by SIRT1 in HepG2 Cells
title_sort galangin induces autophagy via deacetylation of lc3 by sirt1 in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962058/
https://www.ncbi.nlm.nih.gov/pubmed/27460655
http://dx.doi.org/10.1038/srep30496
work_keys_str_mv AT lixv galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells
AT wangyajun galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells
AT xiongyuzhen galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells
AT wujun galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells
AT dinghang galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells
AT chenxiaoyi galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells
AT lanliubo galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells
AT zhanghaitao galangininducesautophagyviadeacetylationoflc3bysirt1inhepg2cells

Ejemplares similares