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Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance
A disintegrin and metallopeptidase with thrombospondin motif type 8 (ADAMTS8), a member of the ADAMTS family, was discovered as a novel angiogenesis inhibitor. We analyzed the expression and methylation of ADAMTS8 in primary gastric tumors and gastric cancer cell lines. We also examined the relation...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963609/ https://www.ncbi.nlm.nih.gov/pubmed/27493958 http://dx.doi.org/10.1155/2016/5083841 |
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author | Chen, Jing Zhang, Jiakui Li, Xin Zhang, Chundong Zhang, Hongbin Jin, Junzhe Dai, Dongqiu |
author_facet | Chen, Jing Zhang, Jiakui Li, Xin Zhang, Chundong Zhang, Hongbin Jin, Junzhe Dai, Dongqiu |
author_sort | Chen, Jing |
collection | PubMed |
description | A disintegrin and metallopeptidase with thrombospondin motif type 8 (ADAMTS8), a member of the ADAMTS family, was discovered as a novel angiogenesis inhibitor. We analyzed the expression and methylation of ADAMTS8 in primary gastric tumors and gastric cancer cell lines. We also examined the relationship between ADAMTS8 expression and methylation and clinicopathologic features. The results showed that the significant downregulation of ADAMTS8 mRNA expression was observed in gastric cancer cell lines and tissues, and its expression was related to invasive depth and lymph node metastasis. CpG was hypermethylated in gastric cancer cell lines MKN45, MGC803, and BGC823, as well as primary gastric cancer specimens. ADAMTS8 mRNA expression was significantly lower in methylated primary gastric tumors. A significant association was found between ADAMTS8 methylation status and lymph node metastasis in primary gastric cancer. Moreover, ADAMTS8 expression was upregulated in the gastric cancer cell lines MGC803, BGC823, and MKN45 after treatment with 5-aza-2′-deoxycytidine. Thus, our results demonstrate that expression of ADAMTS8 mRNA is significantly decreased and DNA methylation is frequent in gastric cancer. ADAMTS8 hypermethylation is associated with decreased expression in gastric cancer and may play an important role in the invasion and metastasis of gastric cancer. |
format | Online Article Text |
id | pubmed-4963609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-49636092016-08-04 Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance Chen, Jing Zhang, Jiakui Li, Xin Zhang, Chundong Zhang, Hongbin Jin, Junzhe Dai, Dongqiu Biomed Res Int Research Article A disintegrin and metallopeptidase with thrombospondin motif type 8 (ADAMTS8), a member of the ADAMTS family, was discovered as a novel angiogenesis inhibitor. We analyzed the expression and methylation of ADAMTS8 in primary gastric tumors and gastric cancer cell lines. We also examined the relationship between ADAMTS8 expression and methylation and clinicopathologic features. The results showed that the significant downregulation of ADAMTS8 mRNA expression was observed in gastric cancer cell lines and tissues, and its expression was related to invasive depth and lymph node metastasis. CpG was hypermethylated in gastric cancer cell lines MKN45, MGC803, and BGC823, as well as primary gastric cancer specimens. ADAMTS8 mRNA expression was significantly lower in methylated primary gastric tumors. A significant association was found between ADAMTS8 methylation status and lymph node metastasis in primary gastric cancer. Moreover, ADAMTS8 expression was upregulated in the gastric cancer cell lines MGC803, BGC823, and MKN45 after treatment with 5-aza-2′-deoxycytidine. Thus, our results demonstrate that expression of ADAMTS8 mRNA is significantly decreased and DNA methylation is frequent in gastric cancer. ADAMTS8 hypermethylation is associated with decreased expression in gastric cancer and may play an important role in the invasion and metastasis of gastric cancer. Hindawi Publishing Corporation 2016 2016-07-14 /pmc/articles/PMC4963609/ /pubmed/27493958 http://dx.doi.org/10.1155/2016/5083841 Text en Copyright © 2016 Jing Chen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Jing Zhang, Jiakui Li, Xin Zhang, Chundong Zhang, Hongbin Jin, Junzhe Dai, Dongqiu Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance |
title | Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance |
title_full | Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance |
title_fullStr | Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance |
title_full_unstemmed | Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance |
title_short | Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance |
title_sort | downregulation of adamts8 by dna hypermethylation in gastric cancer and its clinical significance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963609/ https://www.ncbi.nlm.nih.gov/pubmed/27493958 http://dx.doi.org/10.1155/2016/5083841 |
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