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A method to confer Protein L binding ability to any antibody fragment

Recombinant antibody single-chain variable fragments (scFv) are difficult to purify homogeneously from a protein complex mixture. The most effective, specific and fastest method of purification is an affinity chromatography on Protein L (PpL) matrix. This protein is a multi-domain bacterial surface...

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Detalles Bibliográficos
Autores principales: Lakhrif, Zineb, Pugnière, Martine, Henriquet, Corinne, di Tommaso, Anne, Dimier-Poisson, Isabelle, Billiald, Philippe, Juste, Matthieu O., Aubrey, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966575/
https://www.ncbi.nlm.nih.gov/pubmed/26683650
http://dx.doi.org/10.1080/19420862.2015.1116657