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A method to confer Protein L binding ability to any antibody fragment
Recombinant antibody single-chain variable fragments (scFv) are difficult to purify homogeneously from a protein complex mixture. The most effective, specific and fastest method of purification is an affinity chromatography on Protein L (PpL) matrix. This protein is a multi-domain bacterial surface...
Autores principales: | Lakhrif, Zineb, Pugnière, Martine, Henriquet, Corinne, di Tommaso, Anne, Dimier-Poisson, Isabelle, Billiald, Philippe, Juste, Matthieu O., Aubrey, Nicolas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966575/ https://www.ncbi.nlm.nih.gov/pubmed/26683650 http://dx.doi.org/10.1080/19420862.2015.1116657 |
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