Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration

3-Ketosteroid 9α-hydroxylase (KSH, consisting of KshA and KshB), a key enzyme in steroid metabolism, can catalyze the transformation of 4-androstene-3,17-dione (AD) to 9α-hydroxy-4-androstene-3,17-dione (9OHAD) with NADH as coenzyme. In this work, KSH from Mycobacterium neoaurum JC-12 was successful...

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Detalles Bibliográficos
Autores principales: Zhang, Xian, Rao, Zhiming, Zhang, Lele, Xu, Meijuan, Yang, Taowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967059/
https://www.ncbi.nlm.nih.gov/pubmed/27516945
http://dx.doi.org/10.1186/s40064-016-2871-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967059/
https://www.ncbi.nlm.nih.gov/pubmed/27516945
http://dx.doi.org/10.1186/s40064-016-2871-4

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