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Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli
Ice nucleation protein (INP) is frequently used as a surface anchor for protein display in gram-negative bacteria. Here, MalE and TorA signal peptides, and three charged polypeptides, 6×Lys, 6×Glu and 6×Asp, were anchored to the N-terminus of truncated INP (InaK-N) to improve its surface display eff...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968799/ https://www.ncbi.nlm.nih.gov/pubmed/27479442 http://dx.doi.org/10.1371/journal.pone.0160367 |