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De novo assembly of the blunt snout bream (Megalobrama amblycephala) gill transcriptome to identify ammonia exposure associated microRNAs and their targets

De novo transcriptome sequencing is a robust method for microRNA (miRNA) target gene prediction, especially for organisms without reference genomes. Following exposure of Megalobrama amblycephala to ammonia (0.1 or 20 mg L(−1) ), two cDNA libraries were constructed from the fish gills and sequenced...

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Detalles Bibliográficos
Autores principales: Sun, Shengming, Ge, Xianping, Zhu, Jian, Zhang, Wuxiao, Xuan, Fujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4969262/
https://www.ncbi.nlm.nih.gov/pubmed/27504260
http://dx.doi.org/10.1016/j.rinim.2016.03.001