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Pipeline for amplifying and analyzing amplicons of the V1–V3 region of the 16S rRNA gene

BACKGROUND: Profiling of 16S rRNA gene sequences is an important tool for testing hypotheses in complex microbial communities, and analysis methods must be updated and validated as sequencing technologies advance. In host-associated bacterial communities, the V1–V3 region of the 16S rRNA gene is a v...

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Detalles Bibliográficos
Autores principales: Allen, Heather K., Bayles, Darrell O., Looft, Torey, Trachsel, Julian, Bass, Benjamin E., Alt, David P., Bearson, Shawn M. D., Nicholson, Tracy, Casey, Thomas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970291/
https://www.ncbi.nlm.nih.gov/pubmed/27485508
http://dx.doi.org/10.1186/s13104-016-2172-6