Temperature-Jump Fluorescence Provides Evidence for Fully Reversible Microsecond Dynamics in a Thermophilic Alcohol Dehydrogenase

[Image: see text] Protein dynamics on the microsecond (μs) time scale were investigated by temperature-jump fluorescence spectroscopy as a function of temperature in two variants of a thermophilic alcohol dehydrogenase: W87F and W87F:H43A. Both mutants exhibit a fast, temperature-independent μs decr...

Descripción completa

Detalles Bibliográficos
Autores principales: Meadows, Corey W., Balakrishnan, Gurusamy, Kier, Brandon L., Spiro, Thomas G., Klinman, Judith P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970856/
https://www.ncbi.nlm.nih.gov/pubmed/26223665
http://dx.doi.org/10.1021/jacs.5b04413

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4970856/
https://www.ncbi.nlm.nih.gov/pubmed/26223665
http://dx.doi.org/10.1021/jacs.5b04413

Ejemplares similares