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High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation

The general transcription factor TFIID provides a regulatory platform for transcription initiation. Here we present the crystal structure (1.97 Å) and NMR analysis of yeast TAF1 N-terminal domains TAND1 and TAND2 when bound to yeast TBP, together with mutational data. The yTAF1-TAND1, which in itsel...

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Autores principales: Anandapadamanaban, Madhanagopal, Andresen, Cecilia, Helander, Sara, Ohyama, Yoshifumi, Siponen, Marina I., Lundström, Patrik, Kokubo, Tetsuro, Ikura, Mitsuhiko, Moche, Martin, Sunnerhagen, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972576/
https://www.ncbi.nlm.nih.gov/pubmed/23851461
http://dx.doi.org/10.1038/nsmb.2611
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author Anandapadamanaban, Madhanagopal
Andresen, Cecilia
Helander, Sara
Ohyama, Yoshifumi
Siponen, Marina I.
Lundström, Patrik
Kokubo, Tetsuro
Ikura, Mitsuhiko
Moche, Martin
Sunnerhagen, Maria
author_facet Anandapadamanaban, Madhanagopal
Andresen, Cecilia
Helander, Sara
Ohyama, Yoshifumi
Siponen, Marina I.
Lundström, Patrik
Kokubo, Tetsuro
Ikura, Mitsuhiko
Moche, Martin
Sunnerhagen, Maria
author_sort Anandapadamanaban, Madhanagopal
collection PubMed
description The general transcription factor TFIID provides a regulatory platform for transcription initiation. Here we present the crystal structure (1.97 Å) and NMR analysis of yeast TAF1 N-terminal domains TAND1 and TAND2 when bound to yeast TBP, together with mutational data. The yTAF1-TAND1, which in itself acts as a transcriptional activator, binds into the DNA-binding TBP concave surface by presenting similar anchor residues to TBP as E. coli Mot1 but from a distinct structural scaffold. Furthermore, we show how yTAF1-TAND2 employs an aromatic and acidic anchoring pattern to bind a conserved yTBP surface groove traversing the basic helix region, and we find highly similar TBP-binding motifs also presented by the structurally distinct TFIIA, Mot1 and Brf1 proteins. Our identification of these anchoring patterns, which can be easily disrupted or enhanced, provides compelling insight into the competitive multiprotein TBP interplay critical to transcriptional regulation.
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spelling pubmed-49725762016-08-03 High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation Anandapadamanaban, Madhanagopal Andresen, Cecilia Helander, Sara Ohyama, Yoshifumi Siponen, Marina I. Lundström, Patrik Kokubo, Tetsuro Ikura, Mitsuhiko Moche, Martin Sunnerhagen, Maria Nat Struct Mol Biol Article The general transcription factor TFIID provides a regulatory platform for transcription initiation. Here we present the crystal structure (1.97 Å) and NMR analysis of yeast TAF1 N-terminal domains TAND1 and TAND2 when bound to yeast TBP, together with mutational data. The yTAF1-TAND1, which in itself acts as a transcriptional activator, binds into the DNA-binding TBP concave surface by presenting similar anchor residues to TBP as E. coli Mot1 but from a distinct structural scaffold. Furthermore, we show how yTAF1-TAND2 employs an aromatic and acidic anchoring pattern to bind a conserved yTBP surface groove traversing the basic helix region, and we find highly similar TBP-binding motifs also presented by the structurally distinct TFIIA, Mot1 and Brf1 proteins. Our identification of these anchoring patterns, which can be easily disrupted or enhanced, provides compelling insight into the competitive multiprotein TBP interplay critical to transcriptional regulation. 2013-07-14 2013-08 /pmc/articles/PMC4972576/ /pubmed/23851461 http://dx.doi.org/10.1038/nsmb.2611 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Anandapadamanaban, Madhanagopal
Andresen, Cecilia
Helander, Sara
Ohyama, Yoshifumi
Siponen, Marina I.
Lundström, Patrik
Kokubo, Tetsuro
Ikura, Mitsuhiko
Moche, Martin
Sunnerhagen, Maria
High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
title High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
title_full High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
title_fullStr High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
title_full_unstemmed High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
title_short High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
title_sort high-resolution structure of tbp with taf1 reveals anchoring patterns in transcriptional regulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972576/
https://www.ncbi.nlm.nih.gov/pubmed/23851461
http://dx.doi.org/10.1038/nsmb.2611
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