Optimization of Electrospray Ionization by Statistical Design of Experiments and Response Surface Methodology: Protein–Ligand Equilibrium Dissociation Constant Determinations

Electrospray ionization mass spectrometry (ESI-MS) binding studies between proteins and ligands under native conditions require that instrumental ESI source conditions are optimized if relative solution-phase equilibrium concentrations between the protein–ligand complex and free protein are to be re...

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Detalles Bibliográficos
Autores principales: Pedro, Liliana, Van Voorhis, Wesley C., Quinn, Ronald J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972871/
https://www.ncbi.nlm.nih.gov/pubmed/27225419
http://dx.doi.org/10.1007/s13361-016-1417-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972871/
https://www.ncbi.nlm.nih.gov/pubmed/27225419
http://dx.doi.org/10.1007/s13361-016-1417-x

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