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One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9

Bacteria are indispensable for the study of fundamental molecular biology processes due to their relatively simple gene and genome architecture. The ability to engineer bacterial chromosomes is quintessential for understanding gene functions. Here we demonstrate the engineering of the small-ribosoma...

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Detalles Bibliográficos
Autores principales: Kannan, Krishna, Tsvetanova, Billyana, Chuang, Ray-Yuan, Noskov, Vladimir N., Assad-Garcia, Nacyra, Ma, Li, Hutchison III, Clyde A., Smith, Hamilton O., Glass, John I., Merryman, Chuck, Venter, J. Craig, Gibson, Daniel G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973257/
https://www.ncbi.nlm.nih.gov/pubmed/27489041
http://dx.doi.org/10.1038/srep30714