A simplified method for identifying early CRISPR-induced indels in zebrafish embryos using High Resolution Melting analysis

BACKGROUND: The CRISPR/Cas9 system has become a regularly used tool for editing the genome of many model organisms at specific sites. However, two limiting steps arise in the process of validating guide RNA target sites in larvae and adults: the time required to identify indels and the cost associat...

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Detalles Bibliográficos
Autores principales: Samarut, Éric, Lissouba, Alexandra, Drapeau, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973544/
https://www.ncbi.nlm.nih.gov/pubmed/27491876
http://dx.doi.org/10.1186/s12864-016-2881-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973544/
https://www.ncbi.nlm.nih.gov/pubmed/27491876
http://dx.doi.org/10.1186/s12864-016-2881-1

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