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A simplified method for identifying early CRISPR-induced indels in zebrafish embryos using High Resolution Melting analysis
BACKGROUND: The CRISPR/Cas9 system has become a regularly used tool for editing the genome of many model organisms at specific sites. However, two limiting steps arise in the process of validating guide RNA target sites in larvae and adults: the time required to identify indels and the cost associat...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973544/ https://www.ncbi.nlm.nih.gov/pubmed/27491876 http://dx.doi.org/10.1186/s12864-016-2881-1 |