CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

The site-specific insertion of heterologous genetic material into genomes provides a powerful means to study gene function. Here we describe a modular system entitled CRISPaint (CRISPR-assisted insertion tagging) that allows precise and efficient integration of large heterologous DNA cassettes into...

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Detalles Bibliográficos
Autores principales: Schmid-Burgk, Jonathan L., Höning, Klara, Ebert, Thomas S., Hornung, Veit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974478/
https://www.ncbi.nlm.nih.gov/pubmed/27465542
http://dx.doi.org/10.1038/ncomms12338

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974478/
https://www.ncbi.nlm.nih.gov/pubmed/27465542
http://dx.doi.org/10.1038/ncomms12338

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