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CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
The site-specific insertion of heterologous genetic material into genomes provides a powerful means to study gene function. Here we describe a modular system entitled CRISPaint (CRISPR-assisted insertion tagging) that allows precise and efficient integration of large heterologous DNA cassettes into...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974478/ https://www.ncbi.nlm.nih.gov/pubmed/27465542 http://dx.doi.org/10.1038/ncomms12338 |