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Infection Counter: Automated Quantification of in Vitro Virus Replication by Fluorescence Microscopy

The ability to accurately and reliably quantify viral infection is essential to basic and translational virology research. Here, we describe a simple and robust automated method for using fluorescence microscopy to estimate the proportion of virally infected cells in a monolayer. We provide details...

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Detalles Bibliográficos
Autores principales: Culley, Siân, Towers, Greg J., Selwood, David L., Henriques, Ricardo, Grove, Joe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974536/
https://www.ncbi.nlm.nih.gov/pubmed/27455304
http://dx.doi.org/10.3390/v8070201