Cargando…
Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes
Neutrophil extracellular traps (NETs) have been implicated in the development of certain immune-mediated diseases, but their role in psoriasis has not been clearly defined. Human β-defensin-2 (HBD-2) is an important antimicrobial peptide overexpressed in psoriasis epidermis. We evaluated whether the...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974609/ https://www.ncbi.nlm.nih.gov/pubmed/27493143 http://dx.doi.org/10.1038/srep31119 |
_version_ | 1782446573466157056 |
---|---|
author | Hu, Stephen Chu-Sung Yu, Hsin-Su Yen, Feng-Lin Lin, Chi-Ling Chen, Gwo-Shing Lan, Cheng-Che E. |
author_facet | Hu, Stephen Chu-Sung Yu, Hsin-Su Yen, Feng-Lin Lin, Chi-Ling Chen, Gwo-Shing Lan, Cheng-Che E. |
author_sort | Hu, Stephen Chu-Sung |
collection | PubMed |
description | Neutrophil extracellular traps (NETs) have been implicated in the development of certain immune-mediated diseases, but their role in psoriasis has not been clearly defined. Human β-defensin-2 (HBD-2) is an important antimicrobial peptide overexpressed in psoriasis epidermis. We evaluated whether the amount of NETs is increased in psoriasis and determined the effect of NETs on HBD-2 production in epidermal keratinocytes. Using fluorescent microscopy, we found that patients with psoriasis (n = 48) had higher amount of NETotic cells in their peripheral blood compared to healthy controls (n = 48) and patients with eczema (n = 35). Psoriasis sera showed increased ability to induce NET formation in control neutrophils but normal NET degradation ability. The amount of NETs in the peripheral blood correlated with psoriasis disease severity. NETosis was also observed in the majority (18 of 20) of psoriasis skin specimens. Furthermore, NETs induced HBD-2 mRNA and protein production in keratinocytes, and immunohistochemical analysis confirmed strong expression of HBD-2 in psoriasis lesional skin. In summary, NET formation is increased in peripheral blood and lesional skin of psoriasis patients and correlates with disease severity. Additionally, NET-induced HBD-2 production may provide a novel mechanism for the decreased susceptibility of psoriasis plaques to microbial infections. |
format | Online Article Text |
id | pubmed-4974609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49746092017-01-17 Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes Hu, Stephen Chu-Sung Yu, Hsin-Su Yen, Feng-Lin Lin, Chi-Ling Chen, Gwo-Shing Lan, Cheng-Che E. Sci Rep Article Neutrophil extracellular traps (NETs) have been implicated in the development of certain immune-mediated diseases, but their role in psoriasis has not been clearly defined. Human β-defensin-2 (HBD-2) is an important antimicrobial peptide overexpressed in psoriasis epidermis. We evaluated whether the amount of NETs is increased in psoriasis and determined the effect of NETs on HBD-2 production in epidermal keratinocytes. Using fluorescent microscopy, we found that patients with psoriasis (n = 48) had higher amount of NETotic cells in their peripheral blood compared to healthy controls (n = 48) and patients with eczema (n = 35). Psoriasis sera showed increased ability to induce NET formation in control neutrophils but normal NET degradation ability. The amount of NETs in the peripheral blood correlated with psoriasis disease severity. NETosis was also observed in the majority (18 of 20) of psoriasis skin specimens. Furthermore, NETs induced HBD-2 mRNA and protein production in keratinocytes, and immunohistochemical analysis confirmed strong expression of HBD-2 in psoriasis lesional skin. In summary, NET formation is increased in peripheral blood and lesional skin of psoriasis patients and correlates with disease severity. Additionally, NET-induced HBD-2 production may provide a novel mechanism for the decreased susceptibility of psoriasis plaques to microbial infections. Nature Publishing Group 2016-08-05 /pmc/articles/PMC4974609/ /pubmed/27493143 http://dx.doi.org/10.1038/srep31119 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Hu, Stephen Chu-Sung Yu, Hsin-Su Yen, Feng-Lin Lin, Chi-Ling Chen, Gwo-Shing Lan, Cheng-Che E. Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes |
title | Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes |
title_full | Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes |
title_fullStr | Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes |
title_full_unstemmed | Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes |
title_short | Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes |
title_sort | neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4974609/ https://www.ncbi.nlm.nih.gov/pubmed/27493143 http://dx.doi.org/10.1038/srep31119 |
work_keys_str_mv | AT hustephenchusung neutrophilextracellulartrapformationisincreasedinpsoriasisandinduceshumanbdefensin2productioninepidermalkeratinocytes AT yuhsinsu neutrophilextracellulartrapformationisincreasedinpsoriasisandinduceshumanbdefensin2productioninepidermalkeratinocytes AT yenfenglin neutrophilextracellulartrapformationisincreasedinpsoriasisandinduceshumanbdefensin2productioninepidermalkeratinocytes AT linchiling neutrophilextracellulartrapformationisincreasedinpsoriasisandinduceshumanbdefensin2productioninepidermalkeratinocytes AT chengwoshing neutrophilextracellulartrapformationisincreasedinpsoriasisandinduceshumanbdefensin2productioninepidermalkeratinocytes AT lanchengchee neutrophilextracellulartrapformationisincreasedinpsoriasisandinduceshumanbdefensin2productioninepidermalkeratinocytes |