A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans

Loss-of-function mutagenesis is an important tool used to characterize gene functions, and the CRISPR-Cas9 system is a powerful method for performing targeted mutagenesis in organisms that present low recombination frequencies, such as the serotype D strains of Cryptococcus neoformans. However, when...

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Detalles Bibliográficos
Autores principales: Wang, Yu, Wei, Dongsheng, Zhu, Xiangyang, Pan, Jiao, Zhang, Ping, Huo, Liang, Zhu, Xudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977553/
https://www.ncbi.nlm.nih.gov/pubmed/27503169
http://dx.doi.org/10.1038/srep31145

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4977553/
https://www.ncbi.nlm.nih.gov/pubmed/27503169
http://dx.doi.org/10.1038/srep31145

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