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A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans

To identify genes that regulate the dynamics of lipid droplet (LD) size, we have used the genetically tractable model organism Caenorhabditis elegans, whose wild-type LD population displays a steady state of size with an upper limit of 3 μm in diameter. From a saturated forward genetic screen of 6.7...

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Autores principales: Li, Shiwei, Xu, Shibin, Ma, Yanli, Wu, Shuang, Feng, Yu, Cui, Qingpo, Chen, Lifeng, Zhou, Shuang, Kong, Yuanyuan, Zhang, Xiaoyu, Yu, Jialei, Wu, Mengdi, Zhang, Shaobing O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978895/
https://www.ncbi.nlm.nih.gov/pubmed/27261001
http://dx.doi.org/10.1534/g3.116.030866
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author Li, Shiwei
Xu, Shibin
Ma, Yanli
Wu, Shuang
Feng, Yu
Cui, Qingpo
Chen, Lifeng
Zhou, Shuang
Kong, Yuanyuan
Zhang, Xiaoyu
Yu, Jialei
Wu, Mengdi
Zhang, Shaobing O.
author_facet Li, Shiwei
Xu, Shibin
Ma, Yanli
Wu, Shuang
Feng, Yu
Cui, Qingpo
Chen, Lifeng
Zhou, Shuang
Kong, Yuanyuan
Zhang, Xiaoyu
Yu, Jialei
Wu, Mengdi
Zhang, Shaobing O.
author_sort Li, Shiwei
collection PubMed
description To identify genes that regulate the dynamics of lipid droplet (LD) size, we have used the genetically tractable model organism Caenorhabditis elegans, whose wild-type LD population displays a steady state of size with an upper limit of 3 μm in diameter. From a saturated forward genetic screen of 6.7 × 10(5) mutagenized haploid genomes, we isolated 118 mutants with supersized intestinal LDs often reaching 10 μm. These mutants define nine novel complementation groups, in addition to four known genes (maoc-1, dhs-28, daf-22, and prx-10). The nine groups are named drop (lipid droplet abnormal) and categorized into four classes. Class I mutants drop-5 and drop-9, similar to prx-10, are up-regulated in ACS-22-DGAT-2-dependent LD growth, resistant to LD hydrolysis, and defective in peroxisome import. Class II mutants drop-2, drop-3, drop-6, and drop-7 are up-regulated in LD growth, are resistant to LD hydrolysis, but are not defective in peroxisome import. Class III mutants drop-1 and drop-8 are neither up-regulated in LD growth nor resistant to LD hydrolysis, but seemingly up-regulated in LD fusion. Class IV mutant drop-4 is cloned as sams-1 and, different to the other three classes, is ACS-22-independent and hydrolysis-resistant. These four classes of supersized LD mutants should be valuable for mechanistic studies of LD cellular processes including growth, hydrolysis, and fusion.
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spelling pubmed-49788952016-08-18 A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans Li, Shiwei Xu, Shibin Ma, Yanli Wu, Shuang Feng, Yu Cui, Qingpo Chen, Lifeng Zhou, Shuang Kong, Yuanyuan Zhang, Xiaoyu Yu, Jialei Wu, Mengdi Zhang, Shaobing O. G3 (Bethesda) Investigations To identify genes that regulate the dynamics of lipid droplet (LD) size, we have used the genetically tractable model organism Caenorhabditis elegans, whose wild-type LD population displays a steady state of size with an upper limit of 3 μm in diameter. From a saturated forward genetic screen of 6.7 × 10(5) mutagenized haploid genomes, we isolated 118 mutants with supersized intestinal LDs often reaching 10 μm. These mutants define nine novel complementation groups, in addition to four known genes (maoc-1, dhs-28, daf-22, and prx-10). The nine groups are named drop (lipid droplet abnormal) and categorized into four classes. Class I mutants drop-5 and drop-9, similar to prx-10, are up-regulated in ACS-22-DGAT-2-dependent LD growth, resistant to LD hydrolysis, and defective in peroxisome import. Class II mutants drop-2, drop-3, drop-6, and drop-7 are up-regulated in LD growth, are resistant to LD hydrolysis, but are not defective in peroxisome import. Class III mutants drop-1 and drop-8 are neither up-regulated in LD growth nor resistant to LD hydrolysis, but seemingly up-regulated in LD fusion. Class IV mutant drop-4 is cloned as sams-1 and, different to the other three classes, is ACS-22-independent and hydrolysis-resistant. These four classes of supersized LD mutants should be valuable for mechanistic studies of LD cellular processes including growth, hydrolysis, and fusion. Genetics Society of America 2016-06-01 /pmc/articles/PMC4978895/ /pubmed/27261001 http://dx.doi.org/10.1534/g3.116.030866 Text en Copyright © 2016 Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Li, Shiwei
Xu, Shibin
Ma, Yanli
Wu, Shuang
Feng, Yu
Cui, Qingpo
Chen, Lifeng
Zhou, Shuang
Kong, Yuanyuan
Zhang, Xiaoyu
Yu, Jialei
Wu, Mengdi
Zhang, Shaobing O.
A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans
title A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans
title_full A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans
title_fullStr A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans
title_full_unstemmed A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans
title_short A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans
title_sort genetic screen for mutants with supersized lipid droplets in caenorhabditis elegans
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978895/
https://www.ncbi.nlm.nih.gov/pubmed/27261001
http://dx.doi.org/10.1534/g3.116.030866
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