Genome modifications and cloning using a conjugally transferable recombineering system

The genetic modification of primary bacterial disease isolates is challenging due to the lack of highly efficient genetic tools. Herein we describe the development of a modified PCR-based, λ Red-mediated recombineering system for efficient deletion of genes in Gram-negative bacteria. A series of con...

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Detalles Bibliográficos
Autores principales: Hossain, Mohammad J, Thurlow, Charles M., Sun, Dawei, Nasrin, Shamima, Liles, Mark R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980705/
https://www.ncbi.nlm.nih.gov/pubmed/28352570
http://dx.doi.org/10.1016/j.btre.2015.08.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4980705/
https://www.ncbi.nlm.nih.gov/pubmed/28352570
http://dx.doi.org/10.1016/j.btre.2015.08.005

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