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Globin mRNA reduction for whole-blood transcriptome sequencing
The transcriptome analysis of whole-blood RNA by sequencing holds promise for the identification and tracking of biomarkers; however, the high globin mRNA (gmRNA) content of erythrocytes hampers whole-blood and buffy coat analyses. We introduce a novel gmRNA locking assay (GlobinLock, GL) as a robus...
Autores principales: | Krjutškov, Kaarel, Koel, Mariann, Roost, Anne Mari, Katayama, Shintaro, Einarsdottir, Elisabet, Jouhilahti, Eeva-Mari, Söderhäll, Cilla, Jaakma, Ülle, Plaas, Mario, Vesterlund, Liselotte, Lohi, Hannes, Salumets, Andres, Kere, Juha |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4981843/ https://www.ncbi.nlm.nih.gov/pubmed/27515369 http://dx.doi.org/10.1038/srep31584 |
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