SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix

Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles' heel, it is their potential immunogenicity...

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Detalles Bibliográficos
Autores principales: Boysen, Marta, Schlicksupp, Laura, Dreher, Ingeborg, Loebbert, Ralf, Richter, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983373/
https://www.ncbi.nlm.nih.gov/pubmed/27556050
http://dx.doi.org/10.1155/2016/9096059
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author Boysen, Marta
Schlicksupp, Laura
Dreher, Ingeborg
Loebbert, Ralf
Richter, Mario
author_facet Boysen, Marta
Schlicksupp, Laura
Dreher, Ingeborg
Loebbert, Ralf
Richter, Mario
author_sort Boysen, Marta
collection PubMed
description Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles' heel, it is their potential immunogenicity. To better understand the impact of the size of immune complexes of mAbs on anti-drug antibody (ADA) dependent adverse reactions in Macaca fascicularis, we developed an efficient high-throughput size exclusion chromatography- (SEC-) based methodology that enables analysis of the size, size distribution, and ratio of free and ADA-complexed mAb in serum allowing for assessment of formation and clearance of circulating ADA-mAb immune complexes (CIC).
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spelling pubmed-49833732016-08-23 SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix Boysen, Marta Schlicksupp, Laura Dreher, Ingeborg Loebbert, Ralf Richter, Mario J Immunol Res Research Article Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles' heel, it is their potential immunogenicity. To better understand the impact of the size of immune complexes of mAbs on anti-drug antibody (ADA) dependent adverse reactions in Macaca fascicularis, we developed an efficient high-throughput size exclusion chromatography- (SEC-) based methodology that enables analysis of the size, size distribution, and ratio of free and ADA-complexed mAb in serum allowing for assessment of formation and clearance of circulating ADA-mAb immune complexes (CIC). Hindawi Publishing Corporation 2016 2016-07-31 /pmc/articles/PMC4983373/ /pubmed/27556050 http://dx.doi.org/10.1155/2016/9096059 Text en Copyright © 2016 Marta Boysen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Boysen, Marta
Schlicksupp, Laura
Dreher, Ingeborg
Loebbert, Ralf
Richter, Mario
SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
title SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
title_full SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
title_fullStr SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
title_full_unstemmed SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
title_short SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
title_sort sec based method for size determination of immune complexes of therapeutic antibodies in animal matrix
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983373/
https://www.ncbi.nlm.nih.gov/pubmed/27556050
http://dx.doi.org/10.1155/2016/9096059
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