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Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids
Amino acid analysis is considered to be the gold standard for quantitative peptide and protein analysis. Here, we would like to propose a simple HPLC/UV method based on a reversed-phase separation of the aromatic amino acids tyrosine (Tyr), phenylalanine (Phe), and optionally tryptophan (Trp) withou...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983383/ https://www.ncbi.nlm.nih.gov/pubmed/27559481 http://dx.doi.org/10.1155/2016/7374316 |