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Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP

BACKGROUND: The immunoprecipitation (IP) assay is a valuable molecular biology tool applied across a breadth of fields. The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not easily scaled for high-throughput analysis. RESULTS: Here we describe and charact...

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Detalles Bibliográficos
Autores principales: Mita, Paolo, Lhakhang, Tenzin, Li, Donghui, Eichinger, Daniel J., Fenyo, David, Boeke, Jef D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4983793/
https://www.ncbi.nlm.nih.gov/pubmed/27528826
http://dx.doi.org/10.1186/s12575-016-0046-x