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Functional impact of splice isoform diversity in individual cells

Alternative pre-mRNA splicing provides an effective means for expanding coding capacity of eukaryotic genomes. Recent studies suggest that co-expression of different splice isoforms may increase diversity of RNAs and proteins at a single-cell level. A pertinent question in the field is whether such...

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Detalles Bibliográficos
Autores principales: Yap, Karen, Makeyev, Eugene V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4984447/
https://www.ncbi.nlm.nih.gov/pubmed/27528755
http://dx.doi.org/10.1042/BST20160103
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author Yap, Karen
Makeyev, Eugene V.
author_facet Yap, Karen
Makeyev, Eugene V.
author_sort Yap, Karen
collection PubMed
description Alternative pre-mRNA splicing provides an effective means for expanding coding capacity of eukaryotic genomes. Recent studies suggest that co-expression of different splice isoforms may increase diversity of RNAs and proteins at a single-cell level. A pertinent question in the field is whether such co-expression is biologically meaningful or, rather, represents insufficiently stringent splicing regulation. Here we argue that isoform co-expression may produce functional outcomes that are difficult and sometimes impossible to achieve using other regulation strategies. Far from being a ‘splicing noise’, co-expression is often established through co-ordinated activity of specific cis-elements and trans-acting factors. Further work in this area may uncover new biological functions of alternative splicing (AS) and generate important insights into mechanisms allowing different cell types to attain their unique molecular identities.
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spelling pubmed-49844472016-08-25 Functional impact of splice isoform diversity in individual cells Yap, Karen Makeyev, Eugene V. Biochem Soc Trans Biochemical Society Focused Meetings Alternative pre-mRNA splicing provides an effective means for expanding coding capacity of eukaryotic genomes. Recent studies suggest that co-expression of different splice isoforms may increase diversity of RNAs and proteins at a single-cell level. A pertinent question in the field is whether such co-expression is biologically meaningful or, rather, represents insufficiently stringent splicing regulation. Here we argue that isoform co-expression may produce functional outcomes that are difficult and sometimes impossible to achieve using other regulation strategies. Far from being a ‘splicing noise’, co-expression is often established through co-ordinated activity of specific cis-elements and trans-acting factors. Further work in this area may uncover new biological functions of alternative splicing (AS) and generate important insights into mechanisms allowing different cell types to attain their unique molecular identities. Portland Press Ltd. 2016-08-15 2016-08-15 /pmc/articles/PMC4984447/ /pubmed/27528755 http://dx.doi.org/10.1042/BST20160103 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biochemical Society Focused Meetings
Yap, Karen
Makeyev, Eugene V.
Functional impact of splice isoform diversity in individual cells
title Functional impact of splice isoform diversity in individual cells
title_full Functional impact of splice isoform diversity in individual cells
title_fullStr Functional impact of splice isoform diversity in individual cells
title_full_unstemmed Functional impact of splice isoform diversity in individual cells
title_short Functional impact of splice isoform diversity in individual cells
title_sort functional impact of splice isoform diversity in individual cells
topic Biochemical Society Focused Meetings
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4984447/
https://www.ncbi.nlm.nih.gov/pubmed/27528755
http://dx.doi.org/10.1042/BST20160103
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