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Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions
The dynamic assembly of multi-protein complexes underlies fundamental processes in cell biology. A mechanistic understanding of assemblies requires accurate measurement of their stoichiometry, affinity and cooperativity, and frequently consideration of multiple co-existing complexes. Sedimentation v...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985284/ https://www.ncbi.nlm.nih.gov/pubmed/27436096 http://dx.doi.org/10.7554/eLife.17812 |