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A protein map of the yeast activated spliceosome as obtained by electron microscopy

We have elucidated the spatial arrangement of proteins and snRNP subunits within the purified spliceosomal B(act) complex from Saccharomyces cerevisiae, using negative-stain immunoelectron microscopy. The B(act) spliceosome exhibits a mushroom-like shape with a main body connected to a foot and a st...

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Detalles Bibliográficos
Autores principales: Sun, Chengfu, Rigo, Norbert, Fabrizio, Patrizia, Kastner, Berthold, Lührmann, Reinhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986897/
https://www.ncbi.nlm.nih.gov/pubmed/27368340
http://dx.doi.org/10.1261/rna.057778.116