CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice

Targeted gene disrupted mice can be efficiently generated by expressing a single guide RNA (sgRNA)/CAS9 complex in the zygote. However, the limited success of complicated genome editing, such as large deletions, point mutations, and knockins, remains to be improved. Further, the mosaicism in founder...

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Detalles Bibliográficos
Autores principales: Oji, Asami, Noda, Taichi, Fujihara, Yoshitaka, Miyata, Haruhiko, Kim, Yeon Joo, Muto, Masanaga, Nozawa, Kaori, Matsumura, Takafumi, Isotani, Ayako, Ikawa, Masahito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987700/
https://www.ncbi.nlm.nih.gov/pubmed/27530713
http://dx.doi.org/10.1038/srep31666

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987700/
https://www.ncbi.nlm.nih.gov/pubmed/27530713
http://dx.doi.org/10.1038/srep31666

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