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CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice

Targeted gene disrupted mice can be efficiently generated by expressing a single guide RNA (sgRNA)/CAS9 complex in the zygote. However, the limited success of complicated genome editing, such as large deletions, point mutations, and knockins, remains to be improved. Further, the mosaicism in founder...

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Detalles Bibliográficos
Autores principales:	Oji, Asami, Noda, Taichi, Fujihara, Yoshitaka, Miyata, Haruhiko, Kim, Yeon Joo, Muto, Masanaga, Nozawa, Kaori, Matsumura, Takafumi, Isotani, Ayako, Ikawa, Masahito
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987700/ https://www.ncbi.nlm.nih.gov/pubmed/27530713 http://dx.doi.org/10.1038/srep31666

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