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MRPrimerW: a tool for rapid design of valid high-quality primers for multiple target qPCR experiments

Design of high-quality primers for multiple target sequences is essential for qPCR experiments, but is challenging due to the need to consider both homology tests on off-target sequences and the same stringent filtering constraints on the primers. Existing web servers for primer design have major dr...

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Detalles Bibliográficos
Autores principales: Kim, Hyerin, Kang, NaNa, An, KyuHyeon, Koo, JaeHyung, Kim, Min-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987926/
https://www.ncbi.nlm.nih.gov/pubmed/27154272
http://dx.doi.org/10.1093/nar/gkw380