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Breaking-Cas—interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes

The CRISPR/Cas technology is enabling targeted genome editing in multiple organisms with unprecedented accuracy and specificity by using RNA-guided nucleases. A critical point when planning a CRISPR/Cas experiment is the design of the guide RNA (gRNA), which directs the nuclease and associated machi...

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Detalles Bibliográficos
Autores principales: Oliveros, Juan C., Franch, Mònica, Tabas-Madrid, Daniel, San-León, David, Montoliu, Lluis, Cubas, Pilar, Pazos, Florencio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4987939/
https://www.ncbi.nlm.nih.gov/pubmed/27166368
http://dx.doi.org/10.1093/nar/gkw407