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Use of the melting curve assay as a means for high-throughput quantification of Illumina sequencing libraries

Background. Multiplexed sequencing is commonly performed on massively parallel short-read sequencing platforms such as Illumina, and the efficiency of library normalisation can affect the quality of the output dataset. Although several library normalisation approaches have been established, none are...

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Detalles Bibliográficos
Autores principales: Shinozuka, Hiroshi, Forster, John W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4991867/
https://www.ncbi.nlm.nih.gov/pubmed/27602269
http://dx.doi.org/10.7717/peerj.2281