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Use of the melting curve assay as a means for high-throughput quantification of Illumina sequencing libraries
Background. Multiplexed sequencing is commonly performed on massively parallel short-read sequencing platforms such as Illumina, and the efficiency of library normalisation can affect the quality of the output dataset. Although several library normalisation approaches have been established, none are...
Autores principales: | Shinozuka, Hiroshi, Forster, John W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4991867/ https://www.ncbi.nlm.nih.gov/pubmed/27602269 http://dx.doi.org/10.7717/peerj.2281 |
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