A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein

A simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horse...

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Detalles Bibliográficos
Autores principales: Banasik, Michał, Sachadyn, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992025/
https://www.ncbi.nlm.nih.gov/pubmed/27241123
http://dx.doi.org/10.1007/s12033-016-9949-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992025/
https://www.ncbi.nlm.nih.gov/pubmed/27241123
http://dx.doi.org/10.1007/s12033-016-9949-7

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