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A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein
A simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horse...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992025/ https://www.ncbi.nlm.nih.gov/pubmed/27241123 http://dx.doi.org/10.1007/s12033-016-9949-7 |