Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

The Cas9 endonuclease can be targeted to genomic sequences by programming the sequence of an associated single guide RNA (sgRNA). For unknown reasons, the activity of these Cas9–sgRNA combinations varies widely at different genomic loci and in different cell types. Thus, disrupting genes in polyploi...

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Detalles Bibliográficos
Autores principales: Richardson, C. D., Ray, G. J., Bray, N. L., Corn, J. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992056/
https://www.ncbi.nlm.nih.gov/pubmed/27530320
http://dx.doi.org/10.1038/ncomms12463

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4992056/
https://www.ncbi.nlm.nih.gov/pubmed/27530320
http://dx.doi.org/10.1038/ncomms12463

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