Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Here we outline a next-generation RNA sequencing protocol that enables de novo assemblies and intra-host variant calls of viral genomes collected from clinical and biological sources. The method is unbiased and universal; it uses random primers for cDNA synthesis and requires no prior knowledge of t...

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Detalles Bibliográficos
Autores principales: Matranga, Christian B., Gladden-Young, Adrianne, Qu, James, Winnicki, Sarah, Nosamiefan, Dolo, Levin, Joshua Z., Sabeti, Pardis C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2016
Materias:
Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993327/
https://www.ncbi.nlm.nih.gov/pubmed/27403729
http://dx.doi.org/10.3791/54117

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993327/
https://www.ncbi.nlm.nih.gov/pubmed/27403729
http://dx.doi.org/10.3791/54117

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