Utilisation of ISA Reverse Genetics and Large-Scale Random Codon Re-Encoding to Produce Attenuated Strains of Tick-Borne Encephalitis Virus within Days

Large-scale codon re-encoding is a new method of attenuating RNA viruses. However, the use of infectious clones to generate attenuated viruses has inherent technical problems. We previously developed a bacterium-free reverse genetics protocol, designated ISA, and now combined it with large-scale ran...

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Detalles Bibliográficos
Autores principales: de Fabritus, Lauriane, Nougairède, Antoine, Aubry, Fabien, Gould, Ernest A., de Lamballerie, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993482/
https://www.ncbi.nlm.nih.gov/pubmed/27548676
http://dx.doi.org/10.1371/journal.pone.0159564

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993482/
https://www.ncbi.nlm.nih.gov/pubmed/27548676
http://dx.doi.org/10.1371/journal.pone.0159564

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